| This program computes the concentration of the oligonucletide
on basis of it's sequence and optical density of it's solution.
The short description of this program can be found here. |
Compute oligo Tm
| This program computes the Tm of the oligonucletide on basis
of it's sequence, concentration and concentration of the salt in the
buffer. It uses a nearest-neighbor model for helix propagation. The
nearest-neighbor parameters for predicting DNA duplex stability were
taken from the paper by SantaLucia et al. , Biochemistry, 1996, 35,
3555-3562 (abstract). The sequence should contain only A,C,G,T letters (spaces are OK though) and be 5 to 50 bases long, salt concentration should be 100..1000 mM and oligo concentration - 4..1,000,000 nM. |
SUBTRACT: - a computer program for modeling the process of
subtractive hybridization (abstract).
Download SUBTRACT now -
gzipped Solaris 2.5 binary.