Some Programmettes

Compute oligo concentration
This program computes the concentration of the oligonucletide on basis of it's sequence and optical density of it's solution.

The short description of this program can be found here.

Sequence:

Optical path (cm):
Optical density at 260 nm:

Compute oligo Tm
This program computes the Tm of the oligonucletide on basis of it's sequence, concentration and concentration of the salt in the buffer. It uses a nearest-neighbor model for helix propagation. The nearest-neighbor parameters for predicting DNA duplex stability were taken from the paper by SantaLucia et al. , Biochemistry, 1996, 35, 3555-3562 (abstract).
The sequence should contain only A,C,G,T letters (spaces are OK though) and be 5 to 50 bases long, salt concentration should be 100..1000 mM and oligo concentration - 4..1,000,000 nM.
Sequence:

Oligo Concentration (nM):
Salt concentration (mM):

SUBTRACT: - a computer program for modeling the process of subtractive hybridization (abstract).
Download SUBTRACT now - gzipped Solaris 2.5 binary.